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验证数据展示

  • WB analysis using 66967-2-Ig

    WB analysis using 66967-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66967-2-Ig (GPR116 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

GPR116 Monoclonal antibody

GPR116 Monoclonal Antibody for WB, ELISA
Cat No. 66967-2-Ig

产品说明书

CloneNo. 1H1B2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, ELISA

别名

KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5, ADGRF5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells, MDA-MB-231 cells, Calu-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66967-2-Ig targets GPR116 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5820

Product name: Recombinant human GPR116 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 34-349 aa of BC066121

Sequence: LSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 G protein-coupled receptor 116
别名 KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5, ADGRF5
计算分子量 1346 aa, 149 kDa
观测分子量150 kDa
GenBank蛋白编号BC066121
基因名称 GPR116
Gene ID (NCBI) 221395
RRIDAB_3670377
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IZF2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR116 (ADGRF5, adhesion G protein-coupled receptor F5) is involved in the G protein-coupled receptor signaling pathway and cell surface receptor signaling pathway. may act upstream of or within several processes, including glomerular filtration; pharyngeal arch artery morphogenesis; and surfactant homeostasis.

实验方案

Product Specific Protocols
WB protocol for GPR116 antibody 66967-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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