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验证数据展示

  • IHC staining of human stomach using 20190-1-AP

    IHC staining of human stomach using 20190-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20190-1-AP (GPR105 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human stomach using 20190-1-AP

    IHC staining of human stomach using 20190-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20190-1-AP (GPR105 antibody) at dilution of 1:50 (under 40x lens).

GPR105 Polyclonal antibody

GPR105 Polyclonal Antibody for IHC, ELISA
Cat No. 20190-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

G protein coupled receptor 105, GPR105, KIAA0001, P2RY14, P2Y purinoceptor 14, P2Y14, UDP glucose receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman stomach tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20190-1-AP targets GPR105 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag14105

Product name: Recombinant human GPR105 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 249-338 aa of BC034989

Sequence: YHIARIPYTKSQTEAHYSCQSKEILRYMKEFTLLLSAANVCLDPIIYFFLCQPFREILCKKLHIPLKAQNDLDISRIKRGNTTLESTDTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 purinergic receptor P2Y, G-protein coupled, 14
别名 G protein coupled receptor 105, GPR105, KIAA0001, P2RY14, P2Y purinoceptor 14, P2Y14, UDP glucose receptor
计算分子量 338 aa, 39 kDa
GenBank蛋白编号BC034989
基因名称 P2RY14
Gene ID (NCBI) 9934
RRIDAB_10693612
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15391
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR105 (also known as P2RY14) is widely expressed throughout many brain regions and peripheral tissues of humans and rodents, and couples to a pertussis toxin-sensitive G protein (PMID: 14559350). Notably, GPR105 is also prominently expressed in immune cells, including macrophages, lymphocytes, and neutrophils (PMID: 22778393).

实验方案

Product Specific Protocols
IHC protocol for GPR105 antibody 20190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Mol Brain

Systematic analysis of expression signatures of neuronal subpopulations in the VTA.

Authors - Hyun Jin Kim
View Article