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  • Featured Product
  • KD/KO Validated

GPR103 Polyclonal antibody

GPR103 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25247-1-AP

Print datasheet

Synonyms

AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155

验证数据展示

  • WB analysis of mouse brain using 25247-1-AP

    WB analysis of mouse brain using 25247-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25247-1-AP (GPR103 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25247-1-AP

    IHC staining of human brain using 25247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 25247-1-AP

    IHC staining of human brain using 25247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25247-1-AP is GPR103 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen GPR103 fusion protein Ag19048 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyroglutamylated RFamide peptide receptor
Synonyms AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155
Calculated Molecular Weight 431 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC128133
Gene Symbol QRFPR
Gene ID (NCBI) 84109
RRIDAB_2879987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P65
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR103 antibody 25247-1-APDownload protocol
IHC protocol for GPR103 antibody 25247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Neurochem

N-glycosylation of the human neuropeptide QRFP receptor (QRFPR) is essential for ligand binding and receptor activation.

Authors - Weiwei Wang
View Article
humanWB

J Clin Lab Anal

The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.

Authors - Huanzheng Li
  • KD Validated
View Article