验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
25247-1-AP targets GPR103 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag19048 Product name: Recombinant human QRFPR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 229-356 aa of BC128133 Sequence: EYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNENFKKNVSSAVCYCIVNKTFSPAQRHGNSGITMMRKKAKFSLRENPVEETKGEAFSDGNIEVKLCEQTEEKKKLKRHLALFRSELAENSPLDSGH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyroglutamylated RFamide peptide receptor |
| 别名 | AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155 |
| 计算分子量 | 431 aa, 49 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC128133 |
| 基因名称 | GPR103 |
| Gene ID (NCBI) | 84109 |
| RRID | AB_2879987 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96P65 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR103 antibody 25247-1-AP | Download protocol |
| WB protocol for GPR103 antibody 25247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neurochem N-glycosylation of the human neuropeptide QRFP receptor (QRFPR) is essential for ligand binding and receptor activation. | ||
J Clin Lab Anal The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.
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