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验证数据展示

  • WB analysis of mouse brain using 25247-1-AP

    WB analysis of mouse brain using 25247-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25247-1-AP (GPR103 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25247-1-AP

    IHC staining of human brain using 25247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 25247-1-AP

    IHC staining of human brain using 25247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 40x lens).

  • Featured Product
  • KD/KO验证

GPR103 Polyclonal antibody

GPR103 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25247-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25247-1-AP targets GPR103 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 GPR103 fusion protein Ag19048 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyroglutamylated RFamide peptide receptor
别名 AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155
计算分子量 431 aa, 49 kDa
观测分子量 50 kDa
GenBank蛋白编号BC128133
基因名称 GPR103
Gene ID (NCBI) 84109
RRIDAB_2879987
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96P65
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR103 antibody 25247-1-APDownload protocol
IHC protocol for GPR103 antibody 25247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Neurochem

N-glycosylation of the human neuropeptide QRFP receptor (QRFPR) is essential for ligand binding and receptor activation.

Authors - Weiwei Wang
View Article
humanWB

J Clin Lab Anal

The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.

Authors - Huanzheng Li
  • KD Validated
View Article