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验证数据展示

  • WB analysis of mouse brain using 25247-1-AP

    WB analysis of mouse brain using 25247-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25247-1-AP (GPR103 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25247-1-AP

    IHC staining of human brain using 25247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 25247-1-AP

    IHC staining of human brain using 25247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 40x lens).

  • Featured Product
  • KD/KO验证

GPR103 Polyclonal antibody

GPR103 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25247-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25247-1-AP targets GPR103 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag19048

Product name: Recombinant human QRFPR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 229-356 aa of BC128133

Sequence: EYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNENFKKNVSSAVCYCIVNKTFSPAQRHGNSGITMMRKKAKFSLRENPVEETKGEAFSDGNIEVKLCEQTEEKKKLKRHLALFRSELAENSPLDSGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyroglutamylated RFamide peptide receptor
别名 AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155
计算分子量 431 aa, 49 kDa
观测分子量 50 kDa
GenBank蛋白编号BC128133
基因名称 GPR103
Gene ID (NCBI) 84109
RRIDAB_2879987
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96P65
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR103 antibody 25247-1-APDownload protocol
IHC protocol for GPR103 antibody 25247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Neurochem

N-glycosylation of the human neuropeptide QRFP receptor (QRFPR) is essential for ligand binding and receptor activation.

Authors - Weiwei Wang
View Article
humanWB

J Clin Lab Anal

The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.

Authors - Huanzheng Li
  • KD Validated
View Article