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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84800-1-RR (same clone as 84800-1-PBS)

    WB analysis using 84800-1-RR (same clone as 84800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84800-1-RR (GPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84800-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84800-1-RR (same clone as 84800-1-PBS)

    IF Staining of HepG2 using 84800-1-RR (same clone as 84800-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPD1 antibody (84800-1-RR, Clone: 242359D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84800-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84800-1-RR

    Affinity and Kinetic Characterization of 84800-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84800-1-RR against Human GPD1 were performed. The affinity constant is below 1 pM.

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GPD1 Recombinant monoclonal antibody, PBS Only

GPD1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84800-1-PBS

产品说明书

CloneNo. 242359D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

242359D3, cGPDH, EC:1.1.1.8, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, GPD 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GPD1 Ready-To-Use IHC Kit. GPD1 primary antibody included.

产品信息

84800-1-PBS targets GPD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4278

Product name: Recombinant human GPD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC032234

Sequence: MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycerol-3-phosphate dehydrogenase 1 (soluble)
别名 242359D3, cGPDH, EC:1.1.1.8, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, GPD 1
计算分子量 349 aa, 38 kDa
观测分子量32 kDa
GenBank蛋白编号BC032234
基因名称 GPD1
Gene ID (NCBI) 2819
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21695
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.