验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, mouse liver tissue |
Planning an IHC experiment? We recommend our IHCeasy GPD1 Ready-To-Use IHC Kit. GPD1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
27943-1-AP targets GPD1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag27703 Product name: Recombinant human GPD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 40-181 aa of BC032234 Sequence: VFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycerol-3-phosphate dehydrogenase 1 (soluble) |
| 别名 | GPD C, GPD1, GPDH C |
| 计算分子量 | 349 aa, 38 kDa |
| 观测分子量 | 32-42 kDa |
| GenBank蛋白编号 | BC032234 |
| 基因名称 | GPD1 |
| Gene ID (NCBI) | 2819 |
| RRID | AB_2881016 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21695 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPD1 antibody 27943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Saturation of the mitochondrial NADH shuttles drives aerobic glycolysis in proliferating cells | ||
Mol Cell Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis
|
