验证数据展示
经过测试的应用
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12454-1-AP targets GPAM in IHC, IF/ICC, IP, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | IHC, IF/ICC, IP, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Ag3120 Product name: Recombinant human GPAM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 479-828 aa of BC030783 Sequence: SCAIMSTHIVACLLLYRHRQGIDLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRNDEFFITPSTTVPSVFELNFYSNGVLHVFIMEAIIACSLYAVLNKRGLGGPTSTPPNLISQEQLVRKAASLCYLLSNEGTISLPCQTFYQVCHETVGKFIQYGILTVAEHDDQEDISPSLAEQQWDKKLPEPLSWRSDEEDEDSDFGEEQRDCYLKVSQSKEHQQFITFLQRLLGPLLEAYSSAAIFVHNFSGPVPEPEYLQKLHKYLITRTERNVAVYAESATYCLVKNAVKMFKDIGVFKETKQKRVSVLELSSTFLPQCNRQKLLEYILSFVVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycerol-3-phosphate acyltransferase, mitochondrial |
| 别名 | EC:2.3.1.15, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT 1, GPAT1, GPAT-1 |
| 计算分子量 | 828 aa, 94 kDa |
| 观测分子量 | 90-92 kDa |
| GenBank蛋白编号 | BC030783 |
| 基因名称 | GPAM |
| Gene ID (NCBI) | 57678 |
| RRID | AB_3085394 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HCL2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GPAM antibody 12454-1-AP | Download protocol |
| IHC protocol for GPAM antibody 12454-1-AP | Download protocol |
| IP protocol for GPAM antibody 12454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
