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验证数据展示

  • WB analysis of human peripheral blood platelets using 67271-1-Ig

    WB analysis of human peripheral blood platelets using 67271-1-Ig

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67271-1-Ig

    IHC staining of human spleen using 67271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 67271-1-Ig

    IHC staining of human spleen using 67271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD42a / GP9 Monoclonal antibody

CD42a / GP9 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67271-1-Ig

产品说明书

CloneNo. 2D4H1

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, ELISA

别名

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHuman peripheral blood platelets tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67271-1-Ig targets CD42a / GP9 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag6132

Product name: Recombinant human GP9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-154 aa of BC030229

Sequence: MPAWGALFLLWATAEATKDCPSPCTCRALETMGLWVDCRGHGLTALPALPARTRHLLLANNSLQSVPPGAFDHLPQLQTLDVTQNPWHCDCSLTYLRLWLEDRTPEALLQVRCASPSLAAHGPLGRLTGYQLGSCGWQLQASWVRPGVLWDVAL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glycoprotein IX (platelet)
别名 CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX
计算分子量 19 kDa
观测分子量 20 kDa
GenBank蛋白编号BC030229
基因名称 CD42a
Gene ID (NCBI) 2815
RRIDAB_2882540
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14770
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

实验方案

Product Specific Protocols
IHC protocol for CD42a / GP9 antibody 67271-1-IgDownload protocol
WB protocol for CD42a / GP9 antibody 67271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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