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验证数据展示

  • WB analysis of human peripheral blood platelets using 14564-1-AP

    WB analysis of human peripheral blood platelets using 14564-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 14564-1-AP (CD42a / GP9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 14564-1-AP

    IHC staining of human spleen using 14564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 14564-1-AP

    IHC staining of human spleen using 14564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 40x lens).

CD42a / GP9 Polyclonal antibody

CD42a / GP9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14564-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHuman peripheral blood platelets
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14564-1-AP targets CD42a / GP9 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性mouse
免疫原

CatNo: Ag6129

Product name: Recombinant human GP9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-154 aa of BC030229

Sequence: MPAWGALFLLWATAEATKDCPSPCTCRALETMGLWVDCRGHGLTALPALPARTRHLLLANNSLQSVPPGAFDHLPQLQTLDVTQNPWHCDCSLTYLRLWLEDRTPEALLQVRCASPSLAAHGPLGRLTGYQLGSCGWQLQASWVRPGVLWDVAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycoprotein IX (platelet)
别名 CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX
计算分子量 19 kDa
观测分子量 20 kDa
GenBank蛋白编号BC030229
基因名称 CD42a
Gene ID (NCBI) 2815
RRIDAB_2878067
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14770
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

实验方案

Product Specific Protocols
IHC protocol for CD42a / GP9 antibody 14564-1-APDownload protocol
WB protocol for CD42a / GP9 antibody 14564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Cell Death Dis

Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression

Authors - Rong Yan
View Article
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