验证数据展示

IHC staining of human cerebellum tissue using 24494-1-AP
IHC results of GP-repeated antibody (24494-1-AP, 1:500) with human cerebellum tissue (C9ORF72 expansion carrier) by Prof. Dr. Manuela Neumann. Staining conditions: Ventana automatic staining system using Optiview DAB detection kit; pretreatment boiling for 30min in citrate buffer pH6.
WB analysis of Recombinant protein using 24494-1-AP
Recombinant proteins of SMARCA4 which contains GP repeat sequence were subjected to SDS PAGE followed by western blot with 24494-1-AP (GP repeat Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GP repeat Polyclonal antibody

GP repeat Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24494-1-AP

产品说明书

引用文献(20)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

C9RAN, GGGCC, GP repeat, GPGPGPGP, poly GP, poly PG

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (20)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	Recombinant protein
Positive IHC detected in	human cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 9 publications below
IHC	See 5 publications below
IF	See 6 publications below
ELISA	See 1 publications below

产品信息

24494-1-AP targets GP repeat in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IHC, ELISA Application Description
文献引用应用	WB, IHC, IF, ELISA
经测试反应性	human
文献引用反应性	human, mouse
免疫原	Peptide 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	GP repeat
别名	C9RAN, GGGCC, GP repeat, GPGPGPGP, poly GP, poly PG
基因名称
Gene ID (NCBI)
RRID	AB_2879573
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GP repeated sequence. The recombinant protein 6*His tag-SMARCA4 or GST tag-SMARCA4 which contains the sequence "GPGPGPGPG" were used to detect this antibody.

实验方案

Product Specific Protocols
WB protocol for GP repeat antibody 24494-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Nucleic Acids Res High-throughput screen of 100 000 small molecules in C9ORF72 ALS neurons identifies spliceosome modulators that mobilize G4C2 repeat RNA into nuclear export and repeat associated non-canonical translation Authors - Maartje J Luteijn View Article
human	WB, IHC	Acta Neuropathol Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers. Authors - Ian R A Mackenzie View Article
human	IHC	Acta Neuropathol Increased prevalence of granulovacuolar degeneration in C9orf72 mutation. Authors - Yuichi Riku View Article
human	IF	Acta Neuropathol Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. Authors - Johnathan Cooper-Knock View Article
mouse		EMBO Rep Glucose hypometabolism prompts RAN translation and exacerbates C9orf72-related ALS/FTD phenotypes Authors - Andrew T Nelson View Article
human	WB, IHC	Neuropathol Appl Neurobiol Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins. Authors - Yvonne Davidson View Article

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验证数据展示

IHC staining of human cerebellum tissue using 24494-1-AP

WB analysis of Recombinant protein using 24494-1-AP

GP repeat Polyclonal antibody

GP repeat Polyclonal Antibody for WB, IHC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

High-throughput screen of 100 000 small molecules in C9ORF72 ALS neurons identifies spliceosome modulators that mobilize G4C2 repeat RNA into nuclear export and repeat associated non-canonical translation

Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.

Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.

Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

Glucose hypometabolism prompts RAN translation and exacerbates C9orf72-related ALS/FTD phenotypes

Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.