验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HSC-T6 cells, Jurkat cells, PC-12 cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66134-1-Ig targets GOSR2/Membrin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag21393 Product name: Recombinant human GOSR2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-192 aa of BC009710 Sequence: MDPLFQQTHKQVHEIQSCMGRLETADKQSVHIVENEIQASIDQIFSRLERLEILSSKEPPNKRQNARLRVDQLKYDVQHLQTALRNFQHRRHAREQQERQREELLSRTFTTNDSDTTIPMDESLQFNSSLQKVHNGMDDLILDGHNILDGLRTQRLTLKGTQKKILDIANMLGLSNTVMRLIEKRAFQDKYF 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | golgi SNAP receptor complex member 2 |
| 别名 | 27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin |
| 计算分子量 | 212 aa, 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC009710 |
| 基因名称 | Membrin |
| Gene ID (NCBI) | 9570 |
| RRID | AB_2881533 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O14653 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GOSR2/Membrin antibody 66134-1-Ig | Download protocol |
| IHC protocol for GOSR2/Membrin antibody 66134-1-Ig | Download protocol |
| WB protocol for GOSR2/Membrin antibody 66134-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
