验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, rat testis tissue, HeLa cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy GORASP2 Ready-To-Use IHC Kit. GORASP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10598-1-AP targets GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 | GORASP2 fusion protein Ag0950 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgi reassembly stacking protein 2, 55kDa |
| 别名 | GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC007770 |
| 基因名称 | GORASP2 |
| Gene ID (NCBI) | 26003 |
| RRID | AB_2113473 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H8Y8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IHC protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IF protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IP protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. | ||
Dev Cell A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells.
| ||
J Clin Invest Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy
| ||
J Cell Biol GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.
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