验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse lung tissue, HeLa cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17798-1-AP targets GORAB in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12002 Product name: Recombinant human GORAB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-246 aa of BC064945 Sequence: MSWAAVLAVAAARFGHFWGCRWPGPMAQGWAGFSEEELRRLKQTKDPFEPQRRLPAKKSRQQLQREKALVEQSQKLGLQDGSTSLLPEQLLSAPKQRVNVQKPPFSSPTLPSHFTLTSPVGDGQPQGIESQPKELGLENSHDGHNNVEILPPKPDCKLEKKKVELQEKSRWEVLQQEQRLMEEKNKRKKALLAKAIAERSKRTQAETMKLKRIQKELQALDDMVSADIGILRNRIDQASLDYSYAR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgin, RAB6-interacting |
| 别名 | NTKL binding protein 1, N-terminal kinase-like-binding protein 1, hNTKL-BP1, hNTKL BP1, golgin, RAB6 interacting |
| 计算分子量 | 264aa,28 kDa; 394aa,45 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC064945 |
| 基因名称 | GORAB |
| Gene ID (NCBI) | 92344 |
| RRID | AB_10755145 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T7V8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GORAB, also known as Scyl1BP1, is a protein that localizes to the trans-side of the Golgi apparatus and plays a significant role in intracellular trafficking and protein glycosylation. It is composed of a central coiled-coil region that is responsible for Golgi targeting, likely through interactions with small GTPases Rab6 and Arf5. GORAB is considered a member of the golgin family of coiled-coil Golgi proteins, which are involved in vesicle tethering. GORAB has been implicated in several cellular functions. It has been proposed to function as a transcriptional activator for neurite outgrowth, as a modulator of MDM2 ubiquitylation impacting p53 levels and apoptosis, and has been shown to play a role in centriole duplication and ciliogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GORAB antibody 17798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Gorab Is Required for Dermal Condensate Cells to Respond to Hedgehog Signals during Hair Follicle Morphogenesis.
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FASEB J GORAB promotes embryonic lung maturation through antagonizing AKT phosphorylation, versican expression, and mesenchymal cell migration.
| ||
J Cell Sci Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex | ||
Mol Vis Identification and functional analysis of a novel oculocerebrorenal syndrome of Lowe (OCRL) gene variant in two pedigrees with varying phenotypes including isolated congenital cataract. |
