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验证数据展示

  • WB analysis using 12163-1-AP (same clone as 12163-1-PBS)

    WB analysis using 12163-1-AP (same clone as 12163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12163-1-AP (GOPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12163-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293T using 12163-1-AP (same clone as 12163-1-PBS)

    IF Staining of HEK-293T using 12163-1-AP (same clone as 12163-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using GOPC antibody (12163-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12163-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 12163-1-AP (same clone as 12163-1-PBS)

    IHC staining of mouse kidney using 12163-1-AP (same clone as 12163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12163-1-AP (GOPC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GOPC antibody included
  • IHC staining of mouse kidney using 12163-1-AP (same clone as 12163-1-PBS)

    IHC staining of mouse kidney using 12163-1-AP (same clone as 12163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12163-1-AP (GOPC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GOPC antibody included
  • IHC staining of rat kidney using 12163-1-AP (same clone as 12163-1-PBS)

    IHC staining of rat kidney using 12163-1-AP (same clone as 12163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12163-1-AP (GOPC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GOPC antibody included
  • IF Staining of HEK-293 using 12163-1-AP (same clone as 12163-1-PBS)

    IF Staining of HEK-293 using 12163-1-AP (same clone as 12163-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GOPC antibody (12163-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12163-1-PBS in a different storage buffer formulation.

GOPC Polyclonal antibody, PBS Only

GOPC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12163-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CAL, CFTR-associated ligand, FIG, Golgi-associated PDZ and coiled-coil motif-containing protein, GOPC1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GOPC Ready-To-Use IHC Kit. GOPC primary antibody included.

产品信息

12163-1-PBS targets GOPC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2804

Product name: Recombinant human GOPC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-370 aa of BC009553

Sequence: MSAGGPCPAAAGGGPGGASCSVGAPGGVSMFRWLEVLEKEFDKAFVDVDLLLGEIDPDQADITYEGRQKMTSLSSCFAQLCHKAQSVSQINHKLEAQLVDLKSELTETQAEKVVLEKEVHDQLLQLHSIQLQLHAKTGQSADSGTIKAKLERELEANKKEKMKEAQLEAEVKLLRKENEALRRHIAVLQAEVYGARLAAKYLDKELAGRVQQIQLLGRDMKGPAHDKLWNQLEAEIHLHRHKTVIRACRGRNDLKRPMQAPPGHDQDSLKKSQGVGPIRKVLLLKEDHEGLGISITGGKEHGVPILISEIHPGQPADRCGGLHVGDAILAVNGVNLRDTKHKEAVTILSQQRGEIEFEVVYVAPEVDSDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi associated PDZ and coiled-coil motif containing
别名 CAL, CFTR-associated ligand, FIG, Golgi-associated PDZ and coiled-coil motif-containing protein, GOPC1
计算分子量 454 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC009553
基因名称 GOPC
Gene ID (NCBI) 57120
RRIDAB_2113182
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HD26
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GOPC (FIG/PIST/CAL) is a PDZ-domain scaffolding protein that regulates the trafficking of many integral membrane proteins, including cell surface receptors, ion channels and adhesion molecules. GOPC localizes to the trans-Golgi network (TGN) but not to the cis- or trans-Golgi cisternae, thus anti-GOPC can be used to label the TGN structure.

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