验证数据展示
经过测试的应用
| Positive WB detected in | rat stomach tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31368-1-AP targets GOLT1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag34859 Product name: Recombinant human GOLT1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 95-138 aa of BC012455 Sequence: FLLFRGFFPVVVGFIRRVPVLGSLLNLPGIRSFVDKVGESNNMV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgi transport 1 homolog B (S. cerevisiae) |
| 别名 | CGI 141, GOT1, GOT1A, HDCMA39P, hGOT1a |
| 计算分子量 | 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC012455 |
| 基因名称 | GOLT1B |
| Gene ID (NCBI) | 51026 |
| RRID | AB_3742420 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y3E0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgi transport 1B (GOLT1B) is a member of the GOT1 family, and abnormal expressions of proteins in this family are associated with GA membrane trafficking disorders and cellular malignant behaviors. The GOT1 family consists of two primary members, GOLT1A and GOLT1B. High expression of GOLT1A has been correlated with poor prognosis and resistance to endocrine therapy in breast cancer. GOLT1B is located primarily in early Golgi cisternae, the absence of which induces a substantial reduction in the endoplasmic reticulum (ER)-Golgi transport efficiency. The amplification of GOLT1B is correlated with poor prognosis in lung cancer. GOLT1B contributes to epithelial-mesenchymal transition (EMT) in colorectal cancer (CRC) by activating WNT signaling, influencing the secretion of interferon (IFN)-γ, and apoptosis of tumor-infiltrating T lymphocytes. (PMID: 38130634)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GOLT1B antibody 31368-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
