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GOLGA7 Polyclonal antibody

GOLGA7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14723-1-AP

Print datasheet

Synonyms

GCP16, GOLGA3AP1, GOLGA7, GOLGA7A, Golgin subfamily A member 7, HSPC041

验证数据展示

  • WB analysis using 14723-1-AP

    WB analysis using 14723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14723-1-AP (GOLGA7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 14723-1-AP

    WB analysis of HEK-293 using 14723-1-AP

    WB result of GOLGA7 antibody (14723-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA7 transfected HEK-293 cells.

  • IHC staining of mouse stomach using 14723-1-AP

    IHC staining of mouse stomach using 14723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14723-1-AP (GOLGA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 14723-1-AP

    IHC staining of mouse stomach using 14723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14723-1-AP (GOLGA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HL-60 cells, HepG2 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14723-1-AP targets GOLGA7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen GOLGA7 fusion protein Ag6460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 7
Synonyms GCP16, GOLGA3AP1, GOLGA7, GOLGA7A, Golgin subfamily A member 7, HSPC041
Calculated Molecular Weight 16 kDa
Observed Molecular Weight15-20 kDa
GenBank Accession NumberBC012032
Gene Symbol GOLGA7
Gene ID (NCBI) 51125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z5G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA7 (Golgin subfamily A member 7) is an acylated Golgi protein that interacts with GCP170 protein (PMID: 14522980). The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. The ZDHHC5-GOLGA7 protein acyltransferase complex promotes nonapoptotic cell death (PMID: 31631010).

实验方案

Product Specific Protocols
WB protocol for GOLGA7 antibody 14723-1-APDownload protocol
IHC protocol for GOLGA7 antibody 14723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols