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验证数据展示

  • WB analysis using 14723-1-AP

    WB analysis using 14723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14723-1-AP (GOLGA7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 14723-1-AP

    WB analysis of HEK-293 using 14723-1-AP

    WB result of GOLGA7 antibody (14723-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA7 transfected HEK-293 cells.

  • IHC staining of mouse stomach using 14723-1-AP

    IHC staining of mouse stomach using 14723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14723-1-AP (GOLGA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 14723-1-AP

    IHC staining of mouse stomach using 14723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14723-1-AP (GOLGA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

GOLGA7 Polyclonal antibody

GOLGA7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14723-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GCP16, GOLGA3AP1, GOLGA7, GOLGA7A, Golgin subfamily A member 7, HSPC041

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HL-60 cells, HepG2 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14723-1-AP targets GOLGA7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6460

Product name: Recombinant human GOLGA7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-137 aa of BC012032

Sequence: MRPQQAPVSGKVFIQRDYSSGTRCQFQTKFPAELENRIDRQQFEETVRTLNNLYAEAEKLGGQSYLEGCLACLTAYTIFLCMETHYEKVLKKVSKYIQEQNEKIYAPQGLLLTDPIERGLRVIEITIYEDRGMSSGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 7
别名 GCP16, GOLGA3AP1, GOLGA7, GOLGA7A, Golgin subfamily A member 7, HSPC041
计算分子量 16 kDa
观测分子量15-20 kDa
GenBank蛋白编号BC012032
基因名称 GOLGA7
Gene ID (NCBI) 51125
RRIDAB_3085440
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z5G4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA7 (Golgin subfamily A member 7) is an acylated Golgi protein that interacts with GCP170 protein (PMID: 14522980). The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. The ZDHHC5-GOLGA7 protein acyltransferase complex promotes nonapoptotic cell death (PMID: 31631010).

实验方案

Product Specific Protocols
IHC protocol for GOLGA7 antibody 14723-1-APDownload protocol
WB protocol for GOLGA7 antibody 14723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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