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验证数据展示

  • IF Staining of HeLa using 84955-4-RR

    IF Staining of HeLa using 84955-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA3 antibody (84955-4-RR, Clone: 242603C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • WB analysis using 84955-4-RR

    WB analysis using 84955-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84955-4-RR (GOLGA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84955-4-RR

    Affinity and Kinetic Characterization of 84955-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84955-4-RR against Human GOLGA3 were performed. The affinity constant is 0.475 nM.

GOLGA3 Recombinant monoclonal antibody

GOLGA3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84955-4-RR

产品说明书

CloneNo. 242603C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

GCP170, Golgi complex-associated protein of 170 kDa, Golgin 160, Golgin-160, MEA 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, L02 cells, PC-3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOLGA3 Ready-To-Use IHC Kit. GOLGA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84955-4-RR targets GOLGA3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15531

Product name: Recombinant human GOLGA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC142658

Sequence: MDGASAEQDGLQEDRSHSGPSSLPEAPLKPPGPLVPPDQQDKVQCAEVNRASTEGESPDGPGQGGLCQNGPTPPFPDPPSSLDPTTSPVGPDASPGVAGFHDNLRKSQGTSAEGSVRKEALQSLRLSLPMQETQLCSTDSPLPLEKEEQVRLQARKWLEEQLKQYRVKRQQERSSQPATKTRLFSTLDPELMLNPENLPRASTLAMTKEYSFLRTSVPRGPKVGSLGLPAHPREKKTSKSSKIRSLADYRTEDSNAGNSGGNVPAPDSTKGSLKQNRSSAASVVSEISLSPDTDDRLENTSLAGDSVSEVDGNDSDSSSYSSASTRGTYGILSKTVGTQDTPYMVNGQEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 3
别名 GCP170, Golgi complex-associated protein of 170 kDa, Golgin 160, Golgin-160, MEA 2
计算分子量 1498 aa, 167 kDa
观测分子量 167 kDa
GenBank蛋白编号BC142658
基因名称 GOLGA3
Gene ID (NCBI) 2802
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ08378
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgin subfamily A member 3 (Golga3), a member of the golgin subfamily A, is highly expressed in mouse testis. The GOLGA3 protein, which contains eight phosphorylation sites, is involved in protein transport, cell apoptosis, Golgi localization, and spermatogenesis.

实验方案

Product Specific Protocols
IF protocol for GOLGA3 antibody 84955-4-RRDownload protocol
WB protocol for GOLGA3 antibody 84955-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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