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验证数据展示

  • WB analysis using 84955-4-RR

    WB analysis using 84955-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84955-4-RR (GOLGA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84955-4-RR

    Affinity and Kinetic Characterization of 84955-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84955-4-RR against Human GOLGA3 were performed. The affinity constant is 0.475 nM.

GOLGA3 Recombinant antibody

GOLGA3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84955-4-RR

产品说明书

CloneNo. 242603C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

GCP170, Golgi complex-associated protein of 170 kDa, Golgin 160, Golgin-160, MEA 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, L02 cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy GOLGA3 Ready-To-Use IHC Kit. GOLGA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84955-4-RR targets GOLGA3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 GOLGA3 fusion protein Ag15531 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 3
别名 GCP170, Golgi complex-associated protein of 170 kDa, Golgin 160, Golgin-160, MEA 2
计算分子量 1498 aa, 167 kDa
观测分子量 167 kDa
GenBank蛋白编号BC142658
基因名称 GOLGA3
Gene ID (NCBI) 2802
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ08378
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GOLGA3 antibody 84955-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols