验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human testis tissue, L02 cells, PC-3 cells, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy GOLGA3 Ready-To-Use IHC Kit. GOLGA3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21193-1-AP targets GOLGA3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag15531 Product name: Recombinant human GOLGA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC142658 Sequence: MDGASAEQDGLQEDRSHSGPSSLPEAPLKPPGPLVPPDQQDKVQCAEVNRASTEGESPDGPGQGGLCQNGPTPPFPDPPSSLDPTTSPVGPDASPGVAGFHDNLRKSQGTSAEGSVRKEALQSLRLSLPMQETQLCSTDSPLPLEKEEQVRLQARKWLEEQLKQYRVKRQQERSSQPATKTRLFSTLDPELMLNPENLPRASTLAMTKEYSFLRTSVPRGPKVGSLGLPAHPREKKTSKSSKIRSLADYRTEDSNAGNSGGNVPAPDSTKGSLKQNRSSAASVVSEISLSPDTDDRLENTSLAGDSVSEVDGNDSDSSSYSSASTRGTYGILSKTVGTQDTPYMVNGQEI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgi autoantigen, golgin subfamily a, 3 |
| 别名 | GCP170, Golgi complex-associated protein of 170 kDa, Golgin 160, Golgin-160, MEA 2 |
| 计算分子量 | 1498 aa, 167 kDa |
| 观测分子量 | 167 kDa |
| GenBank蛋白编号 | BC142658 |
| 基因名称 | GOLGA3 |
| Gene ID (NCBI) | 2802 |
| RRID | AB_10733640 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08378 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GOLGA3 antibody 21193-1-AP | Download protocol |
| WB protocol for GOLGA3 antibody 21193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production. | ||
PLoS Biol A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation. | ||
J Biol Chem GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin. | ||
Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. | ||
PeerJ Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis |
