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GNRHR2 Polyclonal antibody

GNRHR2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20728-1-AP

Print datasheet

Synonyms

GnRH II R, GNRHR2

验证数据展示

  • WB analysis of human brain using 20728-1-AP

    WB analysis of human brain using 20728-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20728-1-AP (GNRHR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20728-1-AP

    IHC staining of human kidney using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

  • IF Staining of HEK-293 using 20728-1-AP

    IF Staining of HEK-293 using 20728-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20728-1-AP (GNRHR2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human liver cancer using 20728-1-AP

    IHC staining of human liver cancer using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20728-1-AP (GNRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 20728-1-AP

    IHC staining of human liver cancer using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20728-1-AP (GNRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, human placenta tissue, human stomach tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20728-1-AP targets GNRHR2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gonadotropin-releasing hormone (type 2) receptor 2
Synonyms GnRH II R, GNRHR2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberNR_002328
Gene Symbol GNRHR2
Gene ID (NCBI) 114814
RRIDAB_2878728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GNRHR2 antibody 20728-1-APDownload protocol
IHC protocol for GNRHR2 antibody 20728-1-APDownload protocol
IF protocol for GNRHR2 antibody 20728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols