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验证数据展示

  • WB analysis of HeLa using 66453-1-Ig (same clone as 66453-1-PBS)

    WB analysis of HeLa using 66453-1-Ig (same clone as 66453-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

GNPAT Monoclonal antibody, PBS Only

GNPAT Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66453-1-PBS

产品说明书

CloneNo. 2E5C10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66453-1-PBS targets GNPAT in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 GNPAT fusion protein Ag6740 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glyceronephosphate O-acyltransferase
别名 DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT
计算分子量 77 kDa
观测分子量 65-69 kDa
GenBank蛋白编号BC000450
基因名称 GNPAT
Gene ID (NCBI) 8443
RRIDAB_2881822
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15228
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID: 21990100) This antibody recognize the 65-69 kDa GNPAT protein.