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验证数据展示

  • WB analysis using 67294-1-Ig (same clone as 67294-1-PBS)

    WB analysis using 67294-1-Ig (same clone as 67294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

    IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

GNMT Monoclonal antibody, PBS Only

GNMT Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67294-1-PBS

产品说明书

CloneNo. 1B5E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, Indirect ELISA

别名

EC:2.1.1.20, glycine N methyltransferase, Glycine N-methyltransferase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67294-1-PBS targets GNMT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag4598

Product name: Recombinant human GNMT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-295 aa of BC032627

Sequence: MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLDKDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGSPGLSKFRLSYYPHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKRTD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycine N-methyltransferase
别名 EC:2.1.1.20, glycine N methyltransferase, Glycine N-methyltransferase
计算分子量 295 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC032627
基因名称 GNMT
Gene ID (NCBI) 27232
RRIDAB_2882559
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14749
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

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