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验证数据展示

  • WB analysis using 11988-1-AP

    WB analysis using 11988-1-AP

    Mouse retina tissue were subjected to SDS PAGE followed by western blot with 11988-1-AP (GNGT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 11988-1-AP

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 11988-1-AP

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GNGT2 Polyclonal antibody

GNGT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11988-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GNGT8, GNG9, GNG8, G-gamma-9, G-gamma-8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11988-1-AP targets GNGT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag2618

Product name: Recombinant human GNGT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-69 aa of BC008663

Sequence: MAQDLSEKDLLKMEVEQLKKEVKNTRIPISKAGKEIKEYVEAQAGNDPFLKGIPEDKNPFKEKGGCLIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 2
别名 GNGT8, GNG9, GNG8, G-gamma-9, G-gamma-8
计算分子量 69 aa, 8 kDa
观测分子量 8 kDa
GenBank蛋白编号BC008663
基因名称 GNGT2
Gene ID (NCBI) 2793
RRIDAB_2877813
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14610
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNGT2, also known as GNG8, GNG9 and GNGT8, belongs to the G protein gamma family. Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. This antibody has weak cross-reactivity of GNGT1 protein, as the immunogen of GNGT2 shares about 65% homology with GNGT1.

实验方案

Product Specific Protocols
WB protocol for GNGT2 antibody 11988-1-APDownload protocol
IHC protocol for GNGT2 antibody 11988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Toxicology

C-C motif chemokine ligand 5 contributes to radon exposure-induced lung injury by recruiting dendritic cells to activate effector T helper cells

Authors - Liping Ma
View Article