验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, DU 145 cells, HEK-293 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15781-1-AP targets GNG12 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8470 Product name: Recombinant human GNG12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-72 aa of BC005940 Sequence: MSSKTASTNNIAQARRTVQQLRLEASIEGIKVSKASADLMSYCEEHARSDPLLIGIPTSENPFKDKKTCIIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanine nucleotide binding protein (G protein), gamma 12 |
| 别名 | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 |
| 计算分子量 | 72 aa, 8 kDa |
| 观测分子量 | 10 kDa |
| GenBank蛋白编号 | BC005940 |
| 基因名称 | GNG12 |
| Gene ID (NCBI) | 55970 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBI6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNG12 is a G protein γ subunit (chromosome 1p31.3) that regulates heterotrimeric G protein signaling and plays context-dependent roles in cancer, acting as a tumor suppressor in some cancers but pro-tumorigenic in others.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNG12 antibody 15781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
