• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 15388-1-AP

    WB analysis using 15388-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15388-1-AP (GNB3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15388-1-AP

    IHC staining of human kidney using 15388-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GNB3 Polyclonal antibody

GNB3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15388-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GNB3, Transducin beta chain 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inL02 cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15388-1-AP targets GNB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GNB3 fusion protein Ag7050 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 3
别名 GNB3, Transducin beta chain 3
计算分子量 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC002454
基因名称 GNB3
Gene ID (NCBI) 2784
RRIDAB_2109603
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16520
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

实验方案

Product Specific Protocols
WB protocol for GNB3 antibody 15388-1-APDownload protocol
IHC protocol for GNB3 antibody 15388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols