验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12758-1-AP targets GNAZ in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3255 Product name: Recombinant human GNAZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC026342 Sequence: MGCRQSSEEKEAARRSRRIDRHLRSESQRQRREIKLLLLGTSNSGKSTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADQGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIFCVELSGYDLKLYEDNQTSRMAESLRLFDSICNNNWFINTSLILFLNKKDLLAEKIRRIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEIYSHFTCATDTSNIQFVFDAVTDVIIQNNLKYIGLC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanine nucleotide binding protein (G protein), alpha z polypeptide |
| 别名 | Guanine nucleotide-binding protein G(z) subunit alpha, Gz alpha, Gz-alpha |
| 计算分子量 | 355 aa, 41 kDa |
| 观测分子量 | 38-41 kDa |
| GenBank蛋白编号 | BC026342 |
| 基因名称 | GNAZ |
| Gene ID (NCBI) | 2781 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19086 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanine nucleotide-binding protein G(z) subunit alpha(GNAZ) is a member of a G protein subfamily that mediates signal transduction in pertussis toxin-insensitive systems. Heterotrimeric G proteins consist of alpha (α), beta (β), and gamma (γ) subunits. G proteins act as molecular switches inside cells, functioning to relay and transmit signals from cell surface receptors to intracellular effectors(PMID: 35052777). GNAS transduces signals from G protein-coupled receptors (GPCR) to adenyl cyclase (AC), and finally regulates the expression of cyclic adenosine monophosphate (cAMP)(PMID: 32700107).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAZ antibody 12758-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
