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验证数据展示

  • WB analysis using 68191-1-Ig (same clone as 68191-1-PBS)

    WB analysis using 68191-1-Ig (same clone as 68191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 68191-1-Ig (same clone as 68191-1-PBS)

    WB analysis of MCF-7 using 68191-1-Ig (same clone as 68191-1-PBS)

    WB result of GNAQ antibody (68191-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNAQ transfected MCF-7 cells. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GNAQ Monoclonal antibody, PBS Only

GNAQ Monoclonal Antibody for WB, ELISA
Cat No. 68191-1-PBS

产品说明书

CloneNo. 2H5G6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, ELISA

别名

2H5G6, CMC1, G ALPHA q, GAQ, Guanine nucleotide-binding protein alpha-q

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68191-1-PBS targets GNAQ in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag26178

Product name: Recombinant human GNAQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 84-205 aa of BC057777

Sequence: FTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVD

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), q polypeptide
别名 2H5G6, CMC1, G ALPHA q, GAQ, Guanine nucleotide-binding protein alpha-q
计算分子量 42 kDa
观测分子量 38-40 kDa
GenBank蛋白编号BC057777
基因名称 GNAQ
Gene ID (NCBI) 2776
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP50148
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)