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验证数据展示

  • WB analysis using 68027-1-Ig (same clone as 68027-1-PBS)

    WB analysis using 68027-1-Ig (same clone as 68027-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68027-1-Ig (GNAO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)

    IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51895-1

    Cytometric bead array sample test of MP51895-1

    Cytometric bead array sample test of MP51895-1,GNAO1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68027-1-PBS. Detection antibody: 68027-2-PBS.

  • IF Staining of rat cerebellum using 68027-1-Ig (same clone as 68027-1-PBS)

    IF Staining of rat cerebellum using 68027-1-Ig (same clone as 68027-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using GNAO1 antibody (68027-1-Ig, Clone: 1B5G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51895-1

    Cytometric bead array standard curve of MP51895-1

    Cytometric bead array standard curve of MP51895-1, GNAO1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68027-1-PBS. Detection antibody: 68027-2-PBS. Standard:Ag32045. Range: 1.563-100 ng/mL.

GNAO1 Monoclonal antibody, PBS Only (Capture)

GNAO1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 68027-1-PBS

产品说明书

CloneNo. 1B5G2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit, chicken

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

1B5G2, G ALPHA o, GNAO, Guanine nucleotide-binding protein G(o) subunit alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68027-1-PBS targets GNAO1 as part of a matched antibody pair:

MP51895-1: 68027-1-PBS capture and 68027-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken
免疫原

CatNo: Ag32045

Product name: Recombinant human GNAO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-302 aa of BC030027

Sequence: MKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGPNTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
别名 1B5G2, G ALPHA o, GNAO, Guanine nucleotide-binding protein G(o) subunit alpha
计算分子量 302 aa, 35 kDa
观测分子量40 kDa
GenBank蛋白编号BC030027
基因名称 GNAO1
Gene ID (NCBI) 2775
RRIDAB_2918770
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP09471
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.

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