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GNAI3 Polyclonal antibody

GNAI3 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11641-1-AP

Print datasheet

Synonyms

87U6, G(i) alpha 3, GNAI3

验证数据展示

  • WB analysis using 11641-1-AP

    WB analysis using 11641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 11641-1-AP

    IHC staining of human kidney using 11641-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 11641-1-AP

    IHC staining of human kidney using 11641-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 11641-1-AP

    IF Staining of HepG2 using 11641-1-AP

    Immunofluorescent analysis of HepG2 cells, using GNAI3 antibody 11641-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • FC experiment of A431 using 11641-1-AP

    FC experiment of A431 using 11641-1-AP

    1X10^6 A431 cells were stained with .2ug GNAI3 antibody (11641-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

经过测试的应用

Positive WB detected inrat kidney tissue, A431 cells, mouse brain tissue, human kidney tissue
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11641-1-AP targets GNAI3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen GNAI3 fusion protein Ag2229 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
Synonyms 87U6, G(i) alpha 3, GNAI3
Calculated Molecular Weight 40 kDa
Observed Molecular Weight ~40 kDa
GenBank Accession NumberBC025285
Gene Symbol GNAI3
Gene ID (NCBI) 2773
RRIDAB_2111489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)

实验方案

Product Specific Protocols
WB protocol for GNAI3 antibody 11641-1-APDownload protocol
IHC protocol for GNAI3 antibody 11641-1-APDownload protocol
IF protocol for GNAI3 antibody 11641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Signal

RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.

Authors - Menbere Wendimu
View Article