• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GNA15 Polyclonal antibody

GNA15 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12078-1-AP

Print datasheet

Synonyms

G alpha 15, G alpha 16, G protein subunit alpha 15, G protein subunit alpha 16, GNA15, GNA16

验证数据展示

  • WB analysis using 12078-1-AP

    WB analysis using 12078-1-AP

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 12078-1-AP (GNA15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 12078-1-AP

    IHC staining of human small intestine using 12078-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12078-1-AP (GNA15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMOLT-4 cells, mouse spleen tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12078-1-AP targets GNA15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen GNA15 fusion protein Ag2738 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha 15 (Gq class)
Synonyms G alpha 15, G alpha 16, G protein subunit alpha 15, G protein subunit alpha 16, GNA15, GNA16
Calculated Molecular Weight 374 aa, 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC013585
Gene Symbol GNA15
Gene ID (NCBI) 2769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30679
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNA15, also known as GNA16, belongs to the G-alpha family and G(q) subfamily. The protein encoded by the GNA15 gene is the alpha subunit of the G protein, which participates in the regulation of cell proliferation and apoptosis. GNA15 is highly expressed in some cell types, such as hematopoietic stem cells and epithelial cells during specific stages of differentiation. GNA15 is known to play as an oncogene in several tumors, such as gastroenteropancreatic neuroendocrine neoplasia (GEP-NEN), liver cancer, pancreatic ductal adenocarcinoma and ovarian cancer (PMID: 34552875). The molecular weight of GNA15 is 44 kDa.

实验方案

Product Specific Protocols
WB protocol for GNA15 antibody 12078-1-APDownload protocol
IHC protocol for GNA15 antibody 12078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols