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验证数据展示

  • WB analysis of mouse lung using 13350-1-AP

    WB analysis of mouse lung using 13350-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13350-1-AP (GNA14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 13350-1-AP

    IHC staining of human kidney using 13350-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13350-1-AP (GNA14 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 13350-1-AP

    IHC staining of human kidney using 13350-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13350-1-AP (GNA14 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 13350-1-AP

    IF Staining of HeLa using 13350-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13350-1-AP (GNA14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 13350-1-AP

    IF Staining of HeLa using 13350-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNA14 antibody (13350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GNA14 Polyclonal antibody

GNA14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13350-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Guanine nucleotide-binding protein subunit alpha-14, G-protein subunit alpha-14, G alpha-14

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13350-1-AP targets GNA14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag3930

Product name: Recombinant human GNA14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-355 aa of BC027886

Sequence: MAGCCCLSAEEKESQRISAEIERQLRRDKKDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDRKGFTKLVYQNIFTAMQAMIRAMDTLRIQYVCEQNKENAQIIREVEVDKVSMLSREQVEAIKQLWQDPGIQECYDRRREYQLSDSAKYYLTDIDRIATPSFVPTQQDVLRVRVPTTGIIEYPFDLENIIFRMVDVGGQRSERRKWIHCFESVTSIIFLVALSEYDQVLAECDNENRMEESKALFKTIITYPWFLNSSVILFLNKKDLLEEKIMYSHLISYFPEYTGPKQDVRAARDFILKLYQDQNPDKEKVIYSHFTCATDTDNIRFVFAAVKDTILQLNLREFNLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), alpha 14
别名 Guanine nucleotide-binding protein subunit alpha-14, G-protein subunit alpha-14, G alpha-14
计算分子量 355 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC027886
基因名称 GNA14
Gene ID (NCBI) 9630
RRIDAB_2877942
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95837
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for GNA14 antibody 13350-1-APDownload protocol
IHC protocol for GNA14 antibody 13350-1-APDownload protocol
WB protocol for GNA14 antibody 13350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

GNA14 may be a potential prognostic biomarker in nasopharyngeal carcinoma

Authors - Jinrong Hao
View Article
humanWB,IHC,IF

Cancer Gene Ther

LD-associated signatures identified by perilipin-based proximity labeling proteomics reveal GNA14 as a therapeutic and prognostic target in renal cell carcinoma.

Authors - Yuankang Feng
View Article
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