验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
产品信息
15517-1-AP targets GMPPA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7789 Product name: Recombinant human GMPPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-420 aa of BC007456 Sequence: MLKAVILIGGPQKGTRFRPLSFEVPKPLFPVAGVPMIQHHIEACAQVPGMQEILLIGFYQPDEPLTQFLEAAQQEFNLPVRYLQEFAPLGTGGGLYHFRDQILAGSPEAFFVLNADVCSDFPLSAMLEAHRRQRHPFLLLGTTANRTQSLNYGCIVENPQTHEVLHYVEKPSTFISDIINCGIYLFSPEALKPLRDVFQRNQQDGQLEDSPGLWPGAGTIRLEQDVFSALAGQGQIYVHLTDGIWSQIKSAGSALYASRLYLSRYQDTHPERLAKHTPGGPWIRGNVYIHPTAKVAPSAVLGPNVSIGKGVTVGEGVRLRESIVLHGATLQEHTCVLHSIVGWGSTVGRWARVEGTPSDPNPNDPRARMDSESLFKDGKLLPAITILGCRVRIPAEVLILNSIVLPHKELSRSFTNQIIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GDP-mannose pyrophosphorylase A |
| 别名 | Mannose-1-phosphate guanyltransferase alpha, GTP-mannose-1-phosphate guanylyltransferase alpha, GMPP-alpha, GDP-mannose pyrophosphorylase A, GDP mannose pyrophosphorylase A |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC007456 |
| 基因名称 | GMPPA |
| Gene ID (NCBI) | 29926 |
| RRID | AB_2111056 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96IJ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GMPPA antibody 15517-1-AP | Download protocol |
| IP protocol for GMPPA antibody 15517-1-AP | Download protocol |
| WB protocol for GMPPA antibody 15517-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation. | ||
Front Nutr Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12 | ||
Biomedicines Impact of Hypermannosylation on the Structure and Functionality of the ER and the Golgi Complex
| ||
Front Mol Neurosci Ubiquitination contributes to the regulation of GDP-mannose pyrophosphorylase B activity | ||
J Clin Invest GMPPA defects cause a neuromuscular disorder with α-dystroglycan hyperglycosylation |
