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验证数据展示

  • WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

    IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

Geminin Monoclonal antibody, PBS Only

Geminin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66566-1-PBS

产品说明书

CloneNo. 2E3A2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

GMNN, MGORS6, Gem, 2E3A2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66566-1-PBS targets Geminin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24283

Product name: Recombinant human GMNN protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-209 aa of BC005185

Sequence: MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQYMAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVSSSTDAKPCI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 geminin, DNA replication inhibitor
别名 GMNN, MGORS6, Gem, 2E3A2
计算分子量 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC005185
基因名称 GMNN
Gene ID (NCBI) 51053
RRIDAB_2881927
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75496
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)