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验证数据展示

  • WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

    IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51458-1

    Cytometric bead array sample test of MP51458-1

    Cytometric bead array sample test of MP51458-1, GMNN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66566-1-PBS. Detection antibody: 66566-2-PBS.

  • Cytometric bead array standard curve of MP51458-1

    Cytometric bead array standard curve of MP51458-1

    Cytometric bead array standard curve of MP51458-1, GMNN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66566-1-PBS. Detection antibody: 66566-2-PBS. Standard:Ag24283. Range: 1.563-200 ng/mL.

Geminin Monoclonal antibody, PBS Only (Capture)

Geminin Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66566-1-PBS

产品说明书

CloneNo. 2E3A2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

GMNN, 2E3A2, Gem, MGORS6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66566-1-PBS targets Geminin as part of a matched antibody pair:

MP51458-1: 66566-1-PBS capture and 66566-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24283

Product name: Recombinant human GMNN protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-209 aa of BC005185

Sequence: MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQYMAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVSSSTDAKPCI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 geminin, DNA replication inhibitor
别名 GMNN, 2E3A2, Gem, MGORS6
计算分子量 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC005185
基因名称 GMNN
Gene ID (NCBI) 51053
RRIDAB_2881927
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75496
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

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