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验证数据展示

  • WB analysis using 82705-8-RR

    WB analysis using 82705-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82705-8-RR

    WB analysis of HeLa using 82705-8-RR

    WB result of GOLGA2/GM130 antibody (82705-8-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells.

  • IHC staining of rat kidney using 82705-8-RR

    IHC staining of rat kidney using 82705-8-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLGA2/GM130 antibody included
  • IHC staining of rat kidney using 82705-8-RR

    IHC staining of rat kidney using 82705-8-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLGA2/GM130 antibody included
  • IF Staining of HeLa using 82705-8-RR

    IF Staining of HeLa using 82705-8-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 82705-8-RR

    IF Staining of HeLa using 82705-8-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

  • Affinity and Kinetic Characterization of 82705-8-RR

    Affinity and Kinetic Characterization of 82705-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82705-8-RR against Human GM130/GOLGA2 were performed. The affinity constant is 0.942 nM.

  • Featured Product
  • KD/KO验证

GOLGA2/GM130 Recombinant monoclonal antibody

GOLGA2/GM130 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82705-8-RR

产品说明书

CloneNo. 1M17

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 1M17

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 555
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOLGA2/GM130 Ready-To-Use IHC Kit. GOLGA2/GM130 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8400
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82705-8-RR targets GOLGA2/GM130 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag1848

Product name: Recombinant human GM130;GOLGA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 361-678 aa of BC014188

Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 2
别名 GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 1M17
计算分子量 111 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC014188
基因名称 GM130
Gene ID (NCBI) 2801
RRIDAB_3086513
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ08379
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

实验方案

Product Specific Protocols
WB protocol for GOLGA2/GM130 antibody 82705-8-RRDownload protocol
IHC protocol for GOLGA2/GM130 antibody 82705-8-RRDownload protocol
IF protocol for GOLGA2/GM130 antibody 82705-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols