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验证数据展示

  • FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

    FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

    FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

    1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG(red). 1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98050-1-RR

    Affinity and Kinetic Characterization of 98050-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98050-1-RR against Human GM-CSF were performed. The affinity constant is 3.46 nM.

Anti-Human GM-CSF Rabbit Recombinant Antibody, PBS Only

GM-CSF Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98050-1-PBS

产品说明书

CloneNo. 240183C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

CSF2, 240183C7, Colony stimulating factor, Colony Stimulating Factor 2, Colony-stimulating factor

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98050-1-PBS targets GM-CSF in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg0189

Product name: Recombinant Human GM-CSF protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: N-6*His

Domain: 18-144 aa of

Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 2 (granulocyte-macrophage)
别名 CSF2, 240183C7, Colony stimulating factor, Colony Stimulating Factor 2, Colony-stimulating factor
基因名称 GM-CSF
Gene ID (NCBI) 1437
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP04141
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

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