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验证数据展示

  • WB analysis of human lung using 12426-1-AP

    WB analysis of human lung using 12426-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12426-1-AP (GLYCTK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12426-1-AP

    IHC staining of human pancreas cancer using 12426-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12426-1-AP (GLYCTK antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HeLa using 12426-1-AP

    IF Staining of HeLa using 12426-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLYCTK antibody (12426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

GLYCTK Polyclonal antibody

GLYCTK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12426-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

HBEBP4, HBEBP2, HBeAgBP4A, HBeAg-binding protein 4, GLYCTK1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman lung tissue, human liver tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12426-1-AP targets GLYCTK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3097

Product name: Recombinant human GLYCTK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 151-457 aa of BC021896

Sequence: KALSQLKGGGLAQAAYPAQVVSLILSDVVGDPVEVIASGPTVASSHNVQDCLHILNRYGLRAALPRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVLSAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQIPDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAELRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATFLAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycerate kinase
别名 HBEBP4, HBEBP2, HBeAgBP4A, HBeAg-binding protein 4, GLYCTK1
计算分子量 523 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC021896
基因名称 GLYCTK
Gene ID (NCBI) 132158
RRIDAB_2110802
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVS8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for GLYCTK antibody 12426-1-APDownload protocol
IHC protocol for GLYCTK antibody 12426-1-APDownload protocol
WB protocol for GLYCTK antibody 12426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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