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验证数据展示

  • WB analysis using 20171-1-AP (same clone as 20171-1-PBS)

    WB analysis using 20171-1-AP (same clone as 20171-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20171-1-AP (GLS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20171-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 20171-1-AP (same clone as 20171-1-PBS)

    IHC staining of human liver using 20171-1-AP (same clone as 20171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20171-1-PBS in a different storage buffer formulation.

GLS2 Polyclonal antibody, PBS Only

GLS2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20171-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20171-1-PBS targets GLS2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14003

Product name: Recombinant human GLS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-61 aa of BC059370

Sequence: MRSMKALQKALSRAGSHCGRGGWGHPSRSPLLGGGVRHHLSEAAAQGRETPHSHQPQHQDQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutaminase 2 (liver, mitochondrial)
别名
计算分子量 602 aa, 66 kDa
观测分子量66-70 kDa
GenBank蛋白编号BC059370
基因名称 GLS2
Gene ID (NCBI) 27165
RRIDAB_3085600
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UI32
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLS2, also named as GA and GLS, belongs to the glutaminase family. It is Glutaminase liver isoform . GLS2 plays an important role in the regulation of glutamine catabolism. It promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. GLS2 may play a role in preventing tumor proliferation. This antibody is specific to GLS2.

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