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验证数据展示

  • WB analysis of PBMC cells using 13831-1-AP (same clone as 13831-1-PBS)

    WB analysis of PBMC cells using 13831-1-AP (same clone as 13831-1-PBS)

    WB result of GLRA2 antibody (13831-1-AP, 1:600) with PBMC cells. Proteintech αGLRA2 13831-1-AP in 10% BSA / TBS 0.05% Tween-20 O/N @ 4°C Goat anti-rabbit HRP 1:10000 in 5% milk / TBS 0.05% Tween-20 1h @ RT. This data was developed using the same antibody clone with 13831-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13831-1-AP (same clone as 13831-1-PBS)

    IHC staining of mouse brain using 13831-1-AP (same clone as 13831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13831-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13831-1-AP (same clone as 13831-1-PBS)

    IHC staining of mouse brain using 13831-1-AP (same clone as 13831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13831-1-PBS in a different storage buffer formulation.

  • WB analysis using 13831-1-AP (same clone as 13831-1-PBS)

    WB analysis using 13831-1-AP (same clone as 13831-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13831-1-AP (GLRA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13831-1-PBS in a different storage buffer formulation.

GLRA2 Polyclonal antibody, PBS Only

GLRA2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13831-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13831-1-PBS targets GLRA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4743

Product name: Recombinant human GLRA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-255 aa of BC032864

Sequence: MNRQLVNILTALFAFFLETNHFRTAFCKDHDSRSGKQPSQTLSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSVTETTMDYRVNIFLRQQWNDSRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHDVTTDNKLLRISKNGKVLYSIRLTLTLSCPMDLKNFPMDVQTCTMQLESFGYTMNDLIFEWLSDGPVQVAEGLTLPQFILKEEKELGYCTKHYNTGKFTCIEVKFHLERQMG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycine receptor, alpha 2
别名
计算分子量 452 aa, 52 kDa
观测分子量 48 kDa
GenBank蛋白编号BC032864
基因名称 GLRA2
Gene ID (NCBI) 2742
RRIDAB_2877980
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23416
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

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