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验证数据展示

  • WB analysis of HepG2 using 28272-1-AP

    WB analysis of HepG2 using 28272-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 28272-1-AP

    IHC staining of human colon cancer using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI3 antibody included
  • IHC staining of human prostate cancer using 28272-1-AP

    IHC staining of human prostate cancer using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI3 antibody included
  • IHC staining of mouse testis using 28272-1-AP

    IHC staining of mouse testis using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI3 antibody included
  • IHC staining of rat testis using 28272-1-AP

    IHC staining of rat testis using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLI3 antibody included

GLI3 Polyclonal antibody

GLI3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28272-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GLI3-190, GLI3 full-length protein, GLI3 form of 190 kDa, GLI family zinc finger 3, GCPS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, SKOV-3 cells
Positive IHC detected inmouse testis tissue, human colon cancer tissue, human prostate cancer tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GLI3 Ready-To-Use IHC Kit. GLI3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28272-1-AP targets GLI3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原

CatNo: Ag28472

Product name: Recombinant human GLI3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 641-880 aa of NM_000168

Sequence: MKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GLI family zinc finger 3
别名 GLI3-190, GLI3 full-length protein, GLI3 form of 190 kDa, GLI family zinc finger 3, GCPS
计算分子量 170 aa
观测分子量 170-190 kDa, 80-90 kDa
GenBank蛋白编号NM_000168
基因名称 GLI3
Gene ID (NCBI) 2737
RRIDAB_2881101
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10071
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

实验方案

Product Specific Protocols
WB protocol for GLI3 antibody 28272-1-APDownload protocol
IHC protocol for GLI3 antibody 28272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Bioengineered

All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression

Authors - Quan Hong
View Article
humanWB

Medicine (Baltimore)

miR-200c targeting GLI3 inhibits cell proliferation and promotes apoptosis in non-small cell lung cancer cells

Authors - Xiangjun Yi
View Article