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GLI3 Polyclonal antibody

GLI3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 28272-1-AP

Print datasheet

Synonyms

ACLS, GCPS, GLI family zinc finger 3, GLI3, PAP A, PAPA, PAPA1, PAPB, PHS, PPDIV, Zinc finger protein GLI3

验证数据展示

  • WB analysis of HepG2 using 28272-1-AP

    WB analysis of HepG2 using 28272-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 28272-1-AP

    IHC staining of human colon cancer using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 28272-1-AP

    IHC staining of human prostate cancer using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 28272-1-AP

    IHC staining of mouse testis using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 28272-1-AP

    IHC staining of rat testis using 28272-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, SKOV-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28272-1-AP targets GLI3 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, rat, mouse
Cited Reactivityrat
Immunogen GLI3 fusion protein Ag28472 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GLI family zinc finger 3
Synonyms ACLS, GCPS, GLI family zinc finger 3, GLI3, PAP A, PAPA, PAPA1, PAPB, PHS, PPDIV, Zinc finger protein GLI3
Calculated Molecular Weight 170 aa
Observed Molecular Weight 170-190 kDa, 80-90 kDa
GenBank Accession NumberNM_000168
Gene Symbol GLI3
Gene ID (NCBI) 2737
RRIDAB_2881101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10071
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

实验方案

Product Specific Protocols
WB protocol for GLI3 antibody 28272-1-APDownload protocol
IHC protocol for GLI3 antibody 28272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Bioengineered

All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression

Authors - Quan Hong
View Article