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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84141-1-RR (same clone as 84141-1-PBS)

    WB analysis using 84141-1-RR (same clone as 84141-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84141-1-RR (GLI2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84141-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84141-1-RR (same clone as 84141-1-PBS)

    IF Staining of HepG2 using 84141-1-RR (same clone as 84141-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GLI2-Specific antibody (84141-1-RR, Clone: 241049C10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84141-1-PBS in a different storage buffer formulation.

GLI2-Specific Recombinant antibody, PBS Only

GLI2-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84141-1-PBS

产品说明书

CloneNo. 241049C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Gli2, Tax helper protein, GLI Kruppel family member GLI2, Gli 2, AW546128

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GLI2 Ready-To-Use IHC Kit. GLI2 primary antibody included.

产品信息

84141-1-PBS targets GLI2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GLI family zinc finger 2
别名 Gli2, Tax helper protein, GLI Kruppel family member GLI2, Gli 2, AW546128
计算分子量 168 kDa
观测分子量80-90 kDa, 130-140 kDa, 160-180 kDa
GenBank蛋白编号NM_005270
基因名称 GLI2
Gene ID (NCBI) 2736
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ0VGT2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.