×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 18989-1-AP (same clone as 18989-1-PBS)

    WB analysis using 18989-1-AP (same clone as 18989-1-PBS)

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

    IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLI2 antibody included
  • IF Staining of HepG2 using 18989-1-AP (same clone as 18989-1-PBS)

    IF Staining of HepG2 using 18989-1-AP (same clone as 18989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18989-1-AP (GLI2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GLI2-Specific Polyclonal antibody, PBS Only

GLI2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18989-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy GLI2 Ready-To-Use IHC Kit. GLI2 primary antibody included.

产品信息

18989-1-PBS targets GLI2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GLI family zinc finger 2
别名 Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2
计算分子量 168 kDa
观测分子量167 kDa， 88 kDa
GenBank蛋白编号NM_005270
基因名称 GLI2
Gene ID (NCBI) 14633
RRIDAB_10596479
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10070
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

||
New chat

Able™

正在加载，请稍候...