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  • Featured Product
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Alpha Galactosidase A Monoclonal antibody

Alpha Galactosidase A Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B2C5

Cat No : 66121-1-Ig

Print datasheet

Synonyms

Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha

验证数据展示

  • WB analysis of HEK-293 using 66121-1-Ig

    WB analysis of HEK-293 using 66121-1-Ig

    WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells..

  • WB analysis using 66121-1-Ig

    WB analysis using 66121-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human kidney using 66121-1-Ig

    IHC staining of human kidney using 66121-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 66121-1-Ig

    IHC staining of human kidney using 66121-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 66121-1-Ig

    IF Staining of HepG2 using 66121-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, human kidney tissue, LNCaP cells, A431 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66121-1-Ig targets Alpha Galactosidase A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Alpha Galactosidase A fusion protein Ag7505 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name galactosidase, alpha
Synonyms Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002689
Gene Symbol GLA
Gene ID (NCBI) 2717
RRIDAB_2881520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06280
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

实验方案

Product Specific Protocols
WB protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
IHC protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
IF protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

bioRxiv

Antigen-display exosomes provide adjuvant-free protection against SARS-CoV-2 disease at nanogram levels of spike protein

Authors - Chenxu Guo
View Article
humanWB

Nat Commun

Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis

Authors - Sebastian Boland
View Article