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Alpha galactosidase A Polyclonal antibody

Alpha galactosidase A Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19877-1-AP

Print datasheet

Synonyms

Alpha galactosidase A, galactosidase, alpha

验证数据展示

  • WB analysis of HEK-293 using 19877-1-AP

    WB analysis of HEK-293 using 19877-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19877-1-AP (Alpha galactosidase A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19877-1-AP

    IF Staining of HepG2 using 19877-1-AP

    Immunofluorescent analysis of HepG2 cells, using GLA antibody 19877-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 19877-1-AP is Alpha galactosidase A Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Alpha galactosidase A fusion protein Ag7505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name galactosidase, alpha
Synonyms Alpha galactosidase A, galactosidase, alpha
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002689
Gene Symbol GLA
Gene ID (NCBI) 2717
RRIDAB_10642955
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06280
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLA, also named as Melibiase, Agalsidas and Alpha-galactosidase A, Belongs to the glycosyl hydrolase 27 family. It hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

实验方案

Product Specific Protocols
WB protocol for Alpha galactosidase A antibody 19877-1-APDownload protocol
IF protocol for Alpha galactosidase A antibody 19877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Septin4 promotes cell death in human colon cancer cells by interacting with BAX.

Authors - Xin Zhao
View Article