验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13457-1-AP targets GK5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4269 Product name: Recombinant human GK5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC032470 Sequence: MSGLLTDPEQRAQEPRYPGFVLGLDVGSSVIRCHVYDRAARVCGSSVQKVENLYPQIGWVEIDPDVLWIQFVAVIKEAVKAAGIQMNQIVGLGISTQRATFITWNKKTGNHFHNFISWQDLRAVELVKSWNNSLLMKIFHSSCRVLHFFTRSKRLFTASLFTFTTQQTSLRLVWILQNLTEVQKAVEEENCCFGTIDTWLLYKLTKGSVYATDFSNASTTGLFDPYKMCWSGMITSLISIPLSLLPPVRDTSHNFGSVDEEIFGVPIPIVALLKVPGYDQNICYIFGKGTIEPVFYHGSTL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycerol kinase 5 (putative) |
| 别名 | ATP:glycerol 3-phosphotransferase 5, EC:2.7.1.30, GK 5 |
| 计算分子量 | 529 aa, 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC032470 |
| 基因名称 | GK5 |
| Gene ID (NCBI) | 256356 |
| RRID | AB_2111453 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZS86 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GK5(Putative glycerol kinase 5) is also named as ATP:glycerol 3-phosphotransferase 5 and belongs to the FGGY kinase family.It is involved in Glycerol metabolism and catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate.It has 3 isoforms produced by alternative splicing with the MW of 59 kDa, 34 kDa and 28 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GK5 antibody 13457-1-AP | Download protocol |
| WB protocol for GK5 antibody 13457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
