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GK3P Polyclonal antibody

GK3P Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14176-1-AP

Print datasheet

Synonyms

GK3P, GKP3, GKTB, glycerol kinase 3 pseudogene

验证数据展示

  • WB analysis of HeLa using 14176-1-AP

    WB analysis of HeLa using 14176-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14176-1-AP (GK3P antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 14176-1-AP

    IHC staining of human skin cancer using 14176-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14176-1-AP (GK3P antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin cancer using 14176-1-AP

    IHC staining of human skin cancer using 14176-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14176-1-AP (GK3P antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14176-1-AP targets GK3P in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen GK3P fusion protein Ag5383 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycerol kinase 3 pseudogene
Synonyms GK3P, GKP3, GKTB, glycerol kinase 3 pseudogene
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC066960
Gene Symbol GK3P
Gene ID (NCBI) 2713
RRIDAB_1288669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GK3P antibody 14176-1-APDownload protocol
IHC protocol for GK3P antibody 14176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols