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验证数据展示

  • WB analysis of HeLa cells using 16345-1-AP

    WB analysis of HeLa cells using 16345-1-AP

    WB result from Andre Franz in Hela cell of 16345-1-AP (anti-GINS3).

  • WB analysis of MCF-7 using 16345-1-AP

    WB analysis of MCF-7 using 16345-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16345-1-AP (GINS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16345-1-AP

    IF Staining of HeLa using 16345-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16345-1-AP (GINS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 16345-1-AP

    IHC staining of human ovary cancer using 16345-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16345-1-AP (PSF3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSF3 Polyclonal antibody

PSF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16345-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GINS3, GINS complex subunit 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16345-1-AP targets PSF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSF3 fusion protein Ag9534 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GINS complex subunit 3 (Psf3 homolog)
别名 GINS3, GINS complex subunit 3
计算分子量 216 aa, 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC014437
基因名称 GINS3
Gene ID (NCBI) 64785
RRIDAB_10804660
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSF3 antibody 16345-1-APDownload protocol
IHC protocol for PSF3 antibody 16345-1-APDownload protocol
IF protocol for PSF3 antibody 16345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols