验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
15651-1-AP targets GINS3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8247 Product name: Recombinant human GINS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC005879 Sequence: MSEAYFRVESGALGPEENFLSLDDILMSHEKLPVRTETAMPRLGAFFLERSAGAETDNAVPQGSKLELPLWLAKGLFDNKRRILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDSPENADISQSLLQTFIGRFRRIMDSSQNAYNEDTSALVARLDEMERGLFQTGQKGLNDFQCWEKGQASQITASNLVQNYKKRKFTDMED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GINS complex subunit 3 (Psf3 homolog) |
| 别名 | PSF3, GINS complex subunit 3 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC005879 |
| 基因名称 | GINS3 |
| Gene ID (NCBI) | 64785 |
| RRID | AB_2247477 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRX5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GINS3 (GINS Complex Subunit 3), also known as PSF3, serves as a subunit of the GINS heterotetrameric complex (GINS1/2/3/4). GINS3 is essential for the initiation of DNA replication and progression of replication forks. GINS3 is significantly upregulated in liver hepatocellular carcinoma, where it correlates with poor prognosis, methylation alterations, and immune escape, and has been proposed as a potential diagnostic biomarker.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for GINS3 antibody 15651-1-AP | Download protocol |
| WB protocol for GINS3 antibody 15651-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. | ||
J Clin Invest Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. | ||
JCI Insight Partial loss-of-function mutations in GINS4 lead to NK cell deficiency with neutropenia | ||
Cell Rep Loss of POLE3-POLE4 unleashes replicative gap accumulation upon treatment with PARP inhibitors | ||
Nat Commun Nuclear compartmentalization at the G1/S transition plays a key role in DNA replication control. |
