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验证数据展示

  • WB analysis using 24849-1-AP

    WB analysis using 24849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 24849-1-AP

    IHC staining of human placenta using 24849-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VISTA antibody included
  • IHC staining of human placenta using 24849-1-AP

    IHC staining of human placenta using 24849-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VISTA antibody included
  • IF Staining of human tonsillitis using 24849-1-AP

    IF Staining of human tonsillitis using 24849-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24849-1-AP (VISTA rabbit polyclonal antibody) at dilution of 1:100 and 60181-1-Ig (CD3 mouse monoclonal antibody), further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 24849-1-AP and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60181-1-Ig.

  • IF Staining of human placenta using 24849-1-AP

    IF Staining of human placenta using 24849-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VISTA Polyclonal antibody

VISTA Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 24849-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA and More (1)

别名

C10orf54, B7-H5, Sisp-1, SISP1, PP2135

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, SH-SY5Y cells, human peripheral blood platelets
Positive IHC detected inhuman placenta tissue, human colon cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue, human tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy VISTA Ready-To-Use IHC Kit. VISTA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®532 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24849-1-AP targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 VISTA fusion protein Ag20600 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 54
别名 C10orf54, B7-H5, Sisp-1, SISP1, PP2135
计算分子量 311 aa, 34 kDa
观测分子量45-60 kDa
GenBank蛋白编号BC020568
基因名称 VISTA
Gene ID (NCBI) 64115
RRIDAB_2879755
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7M9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

实验方案

Product Specific Protocols
WB protocol for VISTA antibody 24849-1-APDownload protocol
IHC protocol for VISTA antibody 24849-1-APDownload protocol
IF protocol for VISTA antibody 24849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Nutr Biochem

Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression

Authors - Qi Zhang
View Article
humanIF

Front Cell Dev Biol

Identify the Prognostic and Immune Profile of VSIR in the Tumor Microenvironment: A Pan-Cancer Analysis.

Authors - Yuanyuan Liu
View Article