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验证数据展示

  • WB analysis using 30602-1-AP

    WB analysis using 30602-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30602-1-AP (GHSR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 30602-1-AP

    IHC staining of rat brain using 30602-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30602-1-AP (GHSR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30602-1-AP

    IHC staining of mouse brain using 30602-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30602-1-AP (GHSR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GHSR Polyclonal antibody

GHSR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30602-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GH releasing peptide receptor, GHRP, GHS R

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTT cells
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30602-1-AP targets GHSR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30358

Product name: Recombinant human GHSR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-72 aa of NM_198407

Sequence: MWNATPSEEPGFNLTLADLDWDASPGNDSLGDELLQLFPAPLLAGVTATCVALFVVGIAGNLLTMLVVSRFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth hormone secretagogue receptor
别名 GH releasing peptide receptor, GHRP, GHS R
计算分子量 41 kDa
观测分子量41 kDa
GenBank蛋白编号NM_198407
基因名称 GHSR
Gene ID (NCBI) 2693
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92847
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Growth Hormone Secretagogue Receptor (GHSR), also known as the ghrelin receptor, is a G protein-coupled receptor (GPCR) that plays a crucial role in regulating energy homeostasis, body weight, and growth hormone (GH) release. The GHSR is highly expressed in the brain, and also in some peripheral tissues. GHSR activity is evoked by the stomach-derived peptide hormone ghrelin and abrogated by the intestine-derived liver antimicrobial peptide 2 (LEAP2). (PMID: 33157147)

实验方案

Product Specific Protocols
IHC protocol for GHSR antibody 30602-1-APDownload protocol
WB protocol for GHSR antibody 30602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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