验证数据展示
经过测试的应用
| Positive WB detected in | human saliva, human placenta tissue, rat liver tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:1250-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82813-2-RR targets Growth Hormone in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Eg0383 Product name: recombinant human GH1 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: N-6*HIS Domain: 27-217 aa of BC075012 Sequence: FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | GH1 |
| 别名 | GH, GH1, 4G8, GH 1, GH-N |
| 计算分子量 | 217 aa, 25 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC075012 |
| 基因名称 | GH1 |
| Gene ID (NCBI) | 2688 |
| RRID | AB_3086545 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01241 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Growth Hormone antibody 82813-2-RR | Download protocol |
| WB protocol for Growth Hormone antibody 82813-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |





