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验证数据展示

  • WB analysis using 30148-1-AP

    WB analysis using 30148-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 30148-1-AP (GGT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30148-1-AP

    IHC staining of mouse testis using 30148-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30148-1-AP (GGT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GGT5 Polyclonal antibody

GGT5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30148-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EC:2.3.2.2, EC:3.4.19.13, EC:3.4.19.14, Gamma-glutamyl transpeptidase-related enzyme, Gamma-glutamyltransferase 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in3T3-L1 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30148-1-AP targets GGT5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32373

Product name: Recombinant human GGT5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 387-527 aa of BC011362

Sequence: GTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRTGIILNNELLDLCERCPRGSGTTPSPVSGDRVGGAPGRCWPPVPGERSPSSMVPSILINKAQGSKLVIGGAGGELIISAVAQAIMSKLWLGFDLRAAIAAPILHVNSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gamma-glutamyltransferase 5
别名 EC:2.3.2.2, EC:3.4.19.13, EC:3.4.19.14, Gamma-glutamyl transpeptidase-related enzyme, Gamma-glutamyltransferase 5
计算分子量 586 aa, 62 kDa
观测分子量55-60 kDa
GenBank蛋白编号BC011362
基因名称 GGT5
Gene ID (NCBI) 2687
RRIDAB_3086247
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36269
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gamma-glutamyltransferase 5 (GGT5) is one of the two GGT family members (GGT1 and GGT5) with catalytic activity identified to date and was first reported in 2008 (PMID:18357469). GGT5 is widely distributed in a variety of tissues, with relatively high expression in liver, kidney, and alveolar macrophages (PMID:25377544). GGT5 played a pivotal role in oxidative regulation, drug metabolism, and immune modulation in the human body (PMID:21447318).

实验方案

Product Specific Protocols
WB protocol for GGT5 antibody 30148-1-APDownload protocol
IHC protocol for GGT5 antibody 30148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols