验证数据展示
经过测试的应用
| Positive WB detected in | Calu-1 cells, A549 cells, HT-1080 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32984-1-AP targets GGN in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38372 Product name: Recombinant human GGN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-381 aa of NM_152657.3 Sequence: TESQAGPRNQGQTAGRARGGAPPHAGEGEMAQPADSESGLSLLCKITFKSRPSLAPPAASSSLAAKASLGGGGGGGLFAASGAISYAEVLKQGPLPPGAARPLGEVSRGAQEAEGGDGDGEGCSGPPSAPASQARALPPPPYTTFPGSKPKFDWVSAPDGPERHFRFNGAGGGIGAPRRRAA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gametogenetin |
| 别名 | Gametogenetin |
| 计算分子量 | 67kDa，652aa |
| 观测分子量 | 65 kDa, 70 kDa |
| GenBank蛋白编号 | NM_152657.3 |
| 基因名称 | GGN |
| Gene ID (NCBI) | 199720 |
| RRID | AB_3742813 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q86UU5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human gametogenetin (GGN) is expressed in the testis and ovaries and is a conserved gene in human and mouse. Recently, GGN was found to play a tumor-promoting role in cancer through regulation of NFκB/caspase-3-mediated apoptosis signaling (PMID: 30721393). GGN can be glycosylated, which may result in an increase in observed molecular weight.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GGN antibody 32984-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
